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2020-present

Benoist/Mathis Lab, Department of Immunology, Harvard Medical School, USA

Project 1: Mapping genome-wide association of histone post-translational modifications (H3K4me1, H3K4me3, H3K27me3, H3K36me3, H3K27ac and H3.3) and chromatin-associated proteins (CTCF) in immune cells by using Cleavage Under Targets & Release Using Nuclease (CUT&RUN) as an Immunological Genome (ImmGen) consortium project

  - Spearheaded a collaborative work with EpiCypher, an epigenetics company that launched CUT&RUN, to combat cell population heterogeneity by optimizing their standard CUT&RUN protocol. Success in CUT&RUN optimization and data analysis pipeline were demonstrated in a submitted manuscript
  - Orchestrated data collection from eight ImmGen-participating labs to map chromosome structures across 120 immune cell types


Project 2: Management of ULI RNAseq samples, and communal lab reagents

  - Collaborated with Broad Institute to facilitate 1) generation of ULI RNAseq data produced by SMART- Seq2 sequencing kits in batch-mode and 2) distribution of data to sample owners.
  - Worked on more than 2,500 ULI RNAseq samples from six labs from Harvard Medical School, one lab from Brigham and Women’s Hospital and one lab from University of California San Diego to help produce high quality data, which are used in two publications, six manuscripts in progress and one grant proposal.
  - Organized and ordered common laboratory materials, resulting in lab members being able to conduct experiments on time with all reagents.


2019-2020

Douglas Group, Jenner Institute, University of Oxford, UK

Thesis: A CRISPR-Cas9 screen for hepatocyte receptors for malaria parasite invasion

  - Studied liver stage of malaria by performing high-throughput CRISPR-Cas9 screens to uncover new hepatocyte surface receptors involved in sporozoite invasion, resulting in 1) setting a standard CRISPR-Cas9 screen protocol in studying malaria for general lab use, 2) identification of hepatocyte receptors that play a role in sporozoite invasion and 3) a manuscript in progress.


2017-2018

Watts Lab, Department of Immunology, University of Toronto, Canada

Project 1: Effect of Type I and Type II IFNs on GITR ligand expression in moAPCs
  - Investigated causal transcription factor binding sites for GITR ligand expression in response to type I and II IFNs in RAW264.7, a macrophage-like mouse cell line, using Gaussia-Cypridina dual luciferase reporter assays.


Project 2: Investigation of rs4761847 SNP in *Traf1 using CRISPR-Cas9 to investigate its causal relationship with rheumatoid arthritis.*
  - Designed sgRNA candidates and two repair templates to introduce rs4761847 SNP (A to G) in Traf1 in THP-1 cell-line using CRISPR-Cas9


Summer 2016

Queen Elizabeth II Diamond Jubilee Scholar, Tailor Lab, National Institute of Immunology, India

Project: Interaction between IRF8 and other transcription factors in CD8+ dendritic cell development.
  - Generated lentivirus expressing a plasmid cloned with Irf8-targeting sgRNA and Cas9 from HEK293T. Transduced dendritic cells cultured from Balb/c mice with this lentivirus which resulted in a generation of Irf8^-/-^ cell line for future common lab use.